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Classically Conditioning Earthworms

Worm Apparatus

To condition worms you will need a conditioning tray, paper towel, butanol, rose oil, dechlorinated water, CS syringe, US syringe and gloves

Worm apparatus

To Begin

To begin, cut the paper towel in the shape of the conditioning tray.

Worm tray and paper towel.

Moisten Towel

Moisten the paper towel with dechlorinated water and place in the conditioning tray. This is necessary to keep the worm moist during conditioning.


Tray with wet paper towel inside.

Remove Earthworm

Remove the earthworm from its container and using gloves wash the worm with dechlorinated water. The gloves are necessary when handling the worm because of impurities on the hands of the experimenter.


Earthworm in tray on top of wet paper towel.

Expose Earthworm

Expose earthworm to a CS of rose oil closely followed by a US of butanol. The CS-US interval should be as short as possible (approx.1-2 seconds). The intertrial interval is 1 minute.


Exposing earthworm to rose oil and butanol from syringes

Unconditioned Response

Unconditioned response, worm will retract when exposed to butanol.


Worm retracting from exposure to butanol.

Conditioned Response

Conditioned response to rose oil.


Earthworm's conditioned response to Rose oil

Chart of acquisition and extraction in experiment.


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