Classically Conditioning Earthworms

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Worm Apparatus

To condition worms you will need a conditioning tray, paper towel, butanol, rose oil, dechlorinated water, CS syringe, US syringe and gloves

To Begin

To begin, cut the paper towel in the shape of the conditioning tray.

Moisten Towel

Moisten the paper towel with dechlorinated water and place in the conditioning tray. This is necessary to keep the worm moist during conditioning.

 

Remove Earthworm

Remove the earthworm from its container and using gloves wash the worm with dechlorinated water. The gloves are necessary when handling the worm because of impurities on the hands of the experimenter.

 

Expose Earthworm

Expose earthworm to a CS of rose oil closely followed by a US of butanol. The CS-US interval should be as short as possible (approx.1-2 seconds). The intertrial interval is 1 minute.

 

Unconditioned Response

Unconditioned response, worm will retract when exposed to butanol.

 

Conditioned Response

Conditioned response to rose oil.