How Bacteria Make Decisions

The overall research theme in our lab is bacterial “decision-making”. In other words, what influences a particular bacterial cell to behave in a particular way? What makes genetically identical cells behave differently? How does a cell integrate external and internal information to then enact a particular response? To address such questions, we use two totally distinct model organisms: the non-pathogenic environmental bacterium Bacillus subtilis and the opportunistic human pathogen Pseudomonas aeruginosa. We use molecular genetics, fluorescence microscopy, and a variety of other analytical techniques as appropriate.

B. subtilis and a few other bacterial species use a large protein complex, termed a stressosome, to sense stress and then enact downstream signaling that leads to a response that can vary in its timing and strength. By genetically manipulating strains, we can alter the protein content of the stressosome and then assess the effect of different molecular perturbations on the strength and timing of the subsequent response.

In P. aeruginosa, we study the molecular regulation of pyocins, which are nano-sized, iron atom-tipped spears that can kill other bacterial cells. We also study metabolism and inter-cell communication by this important human pathogen.

ON-RaMP mentees in our lab learn molecular genetics, microscopy, classical microbiological culture, and molecular biology methods. We also have a vibrant lab environment that fosters communication and analytical skills. For more information about our research and our team, please visit Dr. Cabeen's lab site.

Network mentor: Dr. Matthew Cabeen